The diagnostics of autoimmune blister diseases
Autoimmune blister diseases can be divided into two main groups according to the level of blistering, namely intraepidermal blistering formation in pemphigus and subepidermal blistering formation in pemphigoid. The combination of direct and indirect immunofluorescence together with immunoblotting and ELISA research is necessary for a complete diagnosis in autoimmune blister diseases.
The biopsy of the patient's skin or mucosa is an important part of the diagnosis of autoimmune blister diseases. It is best to take the biopsy perilesional, that is from the redness next to the blister. Since immunofluorescence does not work on formalin-fixed material, it is necessary to send a biopsy, preferably in saline solution. This is because saline solution ensures that undesired background is reduced in the staining(see Figure 1). If it is expected that a biopsy will be on the road for longer than 24 hours, it is better to send it on dry ice or in Michel's fixative.
The biopsy is stained for the presence of immunoglobulins and complement, looking at the pattern of staining. With pemphigus, a pattern between the keratinocytes is expected; a so-called honeycomb pattern (Figure 2). Pemphigoid shows a linear pattern along the basal membrane. A distinction can be made here between a linear n-serration pattern (figure 3), suitable for bullous pemphigoid and most other forms of pemphigoid, or a u-serration pattern, suitable for epidermolysis bullosa acquisita (figure 4). Finally, a granular pattern of IgA along the basal membrane can also be found, which is seen in dermatitis herpetiformis (Figure 5).
Together with the findings of the serological examination, we can further classify autoimmune blister diseases. This is important because every form of a blistering disease has a different prognosis and requires different treatment
Serology bullous dermatoses
Serological testing is needed to determine whether circulating antibodies to proteins (antigens) involved in autoimmune blister diseases are present in the patient's blood. This requires a tube of clotted blood from the patient. The combination of serology and biopsy increases the sensitivity and specificity of research into autoimmune blister diseases.
Two substrates are used. In the first place monkey esophagus, where two patterns can be distinguished. The antibodies in pemphigus serum will bind to the epithelial cells, resulting in a chicken wire pattern (Figure 6). In the case of pemphigoid, the antibodies will bind at the site of the basal membrane, giving a linear pattern (Figure 7).
The other substrate is human salt split skin. Incubating the skin of healthy persons with high molar sodium chloride for approximately 24 hours creates a fission at the site of the basal membrane zone. Certain proteins involved in autoimmune blistering diseases (forms of pemphigoid) will be in the roof of the fission (Figure 8), including BP180 and BP230, while other proteins are in the bottom, such as collagen 7 and laminin 332 (Figure 9). Incubation with serum from pemphigoid patients can then give a roof or bottom staining, depending on the antigen to which the antibodies are directed.
With both pemphigus and pemphigoid, it is necessary to perform additional tests, such as ELISA and immunoblot, in order to be able to determine the antigen involved in more detail.
Additional serological tests
Enzyme-Linked Immune Sorbent Assay (ELISA) is a laboratory test that can measure the amount of antibodies in the blood against a certain autoantigen. The advantage of ELISA is that the disease activity can be monitored to see if the medication is effective or if there is a risk of relapse. Our laboratory has anti-desmoglein 1 and anti-desmoglein 3 ELISAs (pemphigus) and anti-NC16A and anti-BP230 ELISAs (pemphigoid).
All sera with a differential diagnosis of pemphigus or pemphigoid are routinely screened with ELISA to see if antibodies to an autoantigen are indeed present. The antibody titer of patients under treatment at the UMCG is checked at each visit.
Immunoblot is a test in which a patient's serum is placed on a nitrocellulose membrane on which skin derived proteins are present. If the serum contains antibodies that are directed against one of these proteins, they will bind to it. This can be made visible with a color method and a diagnosis can be made based on the position where the IgG is bound (Figure 10). With pemphigoid, the technique is particularly suitable for detecting antibodies against BP180 (the antigen that most pemphigoid patients make antibodies to). In addition, it is a very adequate test to detect paraneoplastic pemphigus because it can make periplakine and envoplakine antibodies visible. The initial diagnosis in the event of suspected paraneoplastic pemphigus is immunoblot and indirect immunofluorescence on rat bladder. If the outcome is not clear, the serum is tested by immunoprecipitation. Immunoprecipitation is an extremely sensitive but also laborious test that can detect very low concentrations of antibodies in the blood. Three autoantigens specific to paraneoplastic pemphigus are the combination envoplakine and periplakine and / or the protein A2ML1 (Figure 11). The antibody response may differ per patient.